version 9 5 Search Results


prism version 9.5.1  (GraphPad Software Inc)
90
GraphPad Software Inc prism version 9.5.1
Prism Version 9.5.1, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism version 9.5.1/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism version 9.5.1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pipeline pilot 9.5  (Dassault Systemes)
90
Dassault Systemes pipeline pilot 9.5
Pipeline Pilot 9.5, supplied by Dassault Systemes, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pipeline pilot 9.5/product/Dassault Systemes
Average 90 stars, based on 1 article reviews
pipeline pilot 9.5 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
sas version 9.5  (SAS institute)
90
SAS institute sas version 9.5
Sas Version 9.5, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sas version 9.5/product/SAS institute
Average 90 stars, based on 1 article reviews
sas version 9.5 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
-genomics software version 9.5.3  (CLC Bio)
90
CLC Bio -genomics software version 9.5.3
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Genomics Software Version 9.5.3, supplied by CLC Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/-genomics software version 9.5.3/product/CLC Bio
Average 90 stars, based on 1 article reviews
-genomics software version 9.5.3 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
syngo dynamics software version 9.5  (Siemens AG)
90
Siemens AG syngo dynamics software version 9.5
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Syngo Dynamics Software Version 9.5, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syngo dynamics software version 9.5/product/Siemens AG
Average 90 stars, based on 1 article reviews
syngo dynamics software version 9.5 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
prism version 9.5  (GraphPad Software Inc)
90
GraphPad Software Inc prism version 9.5
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Prism Version 9.5, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism version 9.5/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism version 9.5 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
prism version 9.5.0  (GraphPad Software Inc)
90
GraphPad Software Inc prism version 9.5.0
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Prism Version 9.5.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism version 9.5.0/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism version 9.5.0 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
clc genomics workbench version 9.5.2  (Qiagen)
90
Qiagen clc genomics workbench version 9.5.2
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Clc Genomics Workbench Version 9.5.2, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clc genomics workbench version 9.5.2/product/Qiagen
Average 90 stars, based on 1 article reviews
clc genomics workbench version 9.5.2 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
plot prism graphpad software version 9.5.1  (GraphPad Software Inc)
90
GraphPad Software Inc plot prism graphpad software version 9.5.1
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Plot Prism Graphpad Software Version 9.5.1, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plot prism graphpad software version 9.5.1/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
plot prism graphpad software version 9.5.1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
prism simulator version 9.5.1  (GraphPad Software Inc)
90
GraphPad Software Inc prism simulator version 9.5.1
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Prism Simulator Version 9.5.1, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism simulator version 9.5.1/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism simulator version 9.5.1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
digital workstation syngo dynamic workplace, version 10.0.01_hf04_rev5 [build 2884]  (Siemens Healthineers)
90
Siemens Healthineers digital workstation syngo dynamic workplace, version 10.0.01_hf04_rev5 [build 2884]
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Digital Workstation Syngo Dynamic Workplace, Version 10.0.01 Hf04 Rev5 [Build 2884], supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/digital workstation syngo dynamic workplace, version 10.0.01_hf04_rev5 [build 2884]/product/Siemens Healthineers
Average 90 stars, based on 1 article reviews
digital workstation syngo dynamic workplace, version 10.0.01_hf04_rev5 [build 2884] - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
optimization software gurobi optimization 2022 version 10.0.0  (Gurobi Optimization)
90
Gurobi Optimization optimization software gurobi optimization 2022 version 10.0.0
Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.
Optimization Software Gurobi Optimization 2022 Version 10.0.0, supplied by Gurobi Optimization, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/optimization software gurobi optimization 2022 version 10.0.0/product/Gurobi Optimization
Average 90 stars, based on 1 article reviews
optimization software gurobi optimization 2022 version 10.0.0 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.

Journal: Scientific Reports

Article Title: Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus

doi: 10.1038/s41598-021-03229-4

Figure Lengend Snippet: Sequence of the designed antiviral miRNAs was based on DENV consensus genomic sequence. ( a ) DENV target genes. Through the mariner ( Mos1 ) transposase, ubiquitous and midgut-specific induction promoters drive the expression of a polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. A genomic integration system was used to produce a series of transgenic mosquitoes that can express antiviral miRNAs. The DENV-8miR plasmid contains eight miRNAs: miR_8-1 targeting the 5′UTR, miR_8-7 targeting the 3′UTR, and miR_8-2 to miR_8-6 and miR_8-8 targeting the capsid, as indicated with colored circles. ( b ) The binding site coverage and similarity are presented in the table. Five types of miRNAs for DENV-1 (Myanmar strain), two types of miRNAs for DENV-2 (NGC strain), five types of miRNAs for DENV-3 (98TW503 strain), and two types of miRNAs for DENV-4 (H241 strain) were complementary targets to DENV. The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production. Schematic of the anti-DENV transgene, consisting of an AeCPA / AePUB promoter that drives expression of the polycistronic cluster of eight synthetic small RNAs that were engineered to target conserved genes in the DENV genome. ( d ) Screening of transgenic mosquitoes under fluorescence microscopy. The EGFP reporter driven by the 3 × P3 promoter was expressed in the eyes or anal papillae of transgenic larvae lines. Examples of EGFP reporter expression in larval and adult stages under natural (left) and blue light (right) conditions. Arrows indicate eyes and asterisks indicate anal papillae regions with EGFP expression.

Article Snippet: The viral genome sequences of each serotype were downloaded from the Viral Bioinformatic Resources Center ( http://athena.bioc.uvic.ca/ ) and then aligned using CLC bio-Genomics software version 9.5.3 (CLC Bio, https://www.qiagenbioinformatics.com/ ). ( c ) Schematic of anti-DENV miRNA production.

Techniques: Sequencing, Expressing, Transgenic Assay, Plasmid Preparation, Binding Assay, Software, Fluorescence, Microscopy